Unani system of medicine (Unanipathy) originated in Greece, enriched by Persians and Arabs and now became an integral part of Alternative medicinal systems of India. Itrifal Kishneezi is a Unani medicine prescribed for gastric problems, head ache and used as a stimulant. The present study was taken up as there is no published scientific data available. The drug was tested for antioxidant activity, as there is growing evidence of role of free radicals in disease progression in number of diseases and benefits of concomitant antioxidant administration. Itrifal Kishneezi was tested for DPPH●free radical scavenging and Fe2+ metal ion chelating activity using UV-Vis spectrophotometer. It showed considerable in vitroantioxidant activity in a dose dependent manner.