The hair follicle stem cells are an abundant and easily accessible source of pluripotent adult stem cells. Hairfollicle stem cells differentiate into neurons and glial cells in vitro and express stem cell markers such as tubulin, RIP, P75, and S100. Silibinin, a flavonolignan and the active component of Milk thistle (Silybum Marianum) has been used as a dietary supplement and herbal medication. The aim of the present study was to investigate the effects of Silibinin on hair follicle stem cells differentiation to neural-like cells. The bulge region of the rat whisker was isolated and cultured in Dulbeccos modified Eagles medium supplemented with different concentrations of silibinin and Neurotrophin-3. One week later , bulge cells immunostained with Nestin and p75 and then with tubulin . The morphological and biological features of cultured bulge cells were observed by light microscopy. Present data demonstrated that silibinin (0.5 μg mL-1) can promote differentiation of hair follicle stem cells to neural-like cells.
Maliheh Nobakht, Sara Asalgo, Nahid Rahbar Rooshandel, Kazem Mousavizadeh and Nowrouz Najafzadeh, 2011. Effects of Silibinin on Hair Follicle Stem Cells Differentiation to Neural-like Cells. American Journal of Biochemistry and Molecular Biology, 1: 212-222.