Genetic diversity of Red Junglefowl (Gallus gallus) in five range states of northern India was explored to make conservation action plan for genetically endangered bird species. A total of 111 Red Junglefowl samples were collected and genotyped with 15 polymorphic microsatellite loci. Altogether 173 alleles were found with mean (±SE) allelic number of 11.53±6.96. Loci LEI0234 yielded the highest number of alleles (Na = 32) while loci LEI0166, MCW0222 and MCW0098 yielded the lowest number of alleles (Na = 5) in the population. Out of 15 microsatellite loci used, 14 loci showed PIC value higher than 0.5 and considered informative for population genetic studies. The observed heterozygosity (Ho) in the population ranged between 0.378 and 0.637, with mean (±SE) of 0.391±0.134. Hardy-Weinberg equilibrium (HWE) estimations were conducted and all the 15 loci were found to follow the equation (p>0.05). No evidence for linkage disequilibrium was observed among pair of loci. Genetic bottleneck hypotheses were also tested, suggesting that RJF has not experienced a genetic bottleneck in the recent past. Persent study revealed the considerable genetic variation in this population, which is an important factor for genetic resources conservation, evaluation and for conservation breeding.