The Indian Himalayan Region (IHR) is well known for richness and diversity of valuable but highly sensitive species of medicinal and aromatic plants. Among medicinal plants of the region, genus Berberis has gained an important place in different systems of medicine. Every parts of the species used in medicinal purpose. This genus represents richness of species across Indian Himalayan Region (IHR) with wide ranging distribution from low lying sub-tropical to High alpine areas. The roots stem bark and fruits of various Berberis species in the region are well recognized for their alkaloid contents. In recent years, the global demand for these alkaloids and their derivatives has grown substantially. Its medicinal uses are reported Indian pharmaceutical codex and in different traditional medicines various Analytical tools such as High performance liquid chromatography, Gas chromatography, Gas chromatography and mass spectroscopy infrared spectroscopy progress in natural product chemistry have always liked with innovation in analytical technology. Among the chromatographic techniques High performance thin layer liquids chromatography more popular in quality control and standardization of traditional herbs like Berberis keeping in view of this goal of the present study to develop chromatography fingerprinting of three high value Himalyan Berberis species plant parts. The berberine content in root and stem bark of three Berberis (i.e., B. asiatica, B. aristata, B. lycium) was estimated using a simple High Performance Thin Layer Liquid Chromatography (HPTLC). The comparative methods assessment revealed that the berberine content varied both in root and stem bark samples. More berberine content observed in root samples as compared to bark of all the investigated species. Among the species Berberis asiatica contain more berberine significantly (p<0.05) as compared to B. lycicum and B. aristata.
Harish C. Andola, R.S. Rawal, M.S.M. Rawat, I.D. Bhatt and Vijay Kant Purohit, 2010. Analysis of Berberine Content using HPTLC Fingerprinting of Root and Bark of Three Himalayan Berberis Species. Asian Journal of Biotechnology, 2: 239-245.